Laboratory Development and Shared Antigenicity of Two Prototype Candidate Citrobacter Freundii Vaccines

Cirobacter  freundii are currently standing  as a  newly emerged human pathogen causing several infection types .It may  take an outbreak pattern of  nosocomial  infectious disease. Thus,  a  prototype candidate Citrobacter  freundii  human uro-pathogen were aimed to be  laboratory developed in two versions .The first  was heat killed intact[CFKV] and the second was  stealth live  cell wall defective[CFSLV] vaccines. Both versions  were found to be; safe ,immunogenic  and effective  on  lapin immune and  challenge models. The immune  efficacy  was up to 80% for CF SLV  and 60% for the CFKV vaccines. CFKV and CFSLV  vaccines  were with no mortality but with mild short lasting  morbidity. The criteria of the  laboratory developed  C. fruendii vaccines were matching that of typhoid vaccine Ty21a.The stealth FCSLV of  freundii vaccine has shown shared  antigenicity with CFKV vaccine version. Such shared antigenicity was found to be of bilateral nature  both in quantitative and qualitative terms. CFKV and CFSLV vaccines  induced humoral antibody responses  which  may be of Th2 dependent B cell responses. T cell responses are far from being operable in  the immune  efficacy of these vaccines, since  C .fruendii are neither obligate nor facultative intracellular parasites . Vaccine lapin  cross challenge models  are waiting to be explored in a  future work

Fifth Generation (5G) New Radio (NR) Channel Codes Contenders Based on Field- Programmable Gate Arrays (FPGA): A Review Paper

ان الحاجة المتزايدة على الجودة، مثل السرعة العالية والتاخير المنخفض والتغطية الواسعة واستهلاك الطاقة والتكلفة والاتصالات الموثوقة في خدمات الهاتف المحمول والوسائط المتعددة ونقل البيانات تفرض استخدام المتطلبات التقنية المتقدمة في الجيل الخامس (5G) الإذاعة الجديدة (NR). واحدة من أهم الأجزاء في الطبقة المادية للجيل الجديد هي تقنية الترميز لتصحيح الأخطاء. هنالك ثلاثة اشكال مقترحة لتقنيات الترميز المخصصة لقنوات نقل البيانات وقنوات التحكم هي  الترميز التوربيني وفحص التكافؤ المنخفض الكثافة (LDPC) والرموز القطبية. يتم تقييم المنافسة بين هذه الانواع من حيث القدرة على تصحيح الأخطاء والتعقيد الحسابي والمرونة. التوازي والمرونة وسرعة المعالجة العالية لمصفوفة البوابة القابلة للبرمجة الميدانية (FPGA) تجعلها أفضل في النماذج الأولية وتنفيذ الرموز المختلفة. تقدم هذه الورقة دراسة استقصائية للبحوث الحالية التي تتعامل مع تصميم وحدة فك الترميز المستندة إلى FPGA المرتبطة برموز القناة المذكورة سابقًا.The increased demands for quality, like high throughput, low-latency, wide coverage, energy consumption, cost and reliable connections in mobile services, multimedia and data transmission impose the use of advance technical requirements for the next fifth-generation (5G) new radio (NR). One of the most crucial parts in the physical layer of the new generation is the error correction coding technique. Three schemes, namely; Turbo, low density parity check (LDPC), and polar codes are potentially ‎considered as the candidate codes for both data and control channels. The competition is evaluated in terms of error correction capability, computational complexity, and flexibility. The parallelism, flexibility and high processing speed of Field-Programmable Gate Array (FPGA) make it preferable in prototyping and implementation of different codes. This paper presents a survey on the current literatures that deals with FPGA-based decoder design associated with the previously mentioned channel codes

Carbapenem resistance expressed by Gram-negative bacilli isolated from a cohort of Libyan patients

Background and objectives: Carbapenem-resistant Enterobacteriaceae (CRE) and other Gram-negative bacteria are among the most common pathogens responsible for both community and hospital acquired infection. The global spread of cephalosporinases in Enterobacteriaceae has led to the increased use of carbapenems resulting in the emergence and rapid spread of CRE. This has become an alarming public health concern, yet the condition in Libya remains unclear. The aim of this study was to obtain a better understanding of CRE strains prevalent in Libyan patients by investigating their phenotypic characteristics and antibiograms. Methods: Gram-negative bacterial species were collected from Misrata Central Hospital, Misrata Cancer Centre and Privet Pathology Laboratories. Clinical samples and swabs were obtained from hospitalised and non-hospitalised patients and from mechanical ventilation and suction machines. Patients who had received antibiotic therapy for at least three days prior to the study were excluded. The identification and characterization of the isolated species were achieved using the growth characteristics on MacConkey and blood agar, spot tests and API 20E or API 20NE biochemical testing systems. Screening for carbapenem resistance was performed using the disk diffusion method with carbapenem 10 μg and cephalosporin 30 μg disks and minimum inhibitory concentrations (MIC) determined using the Sensititre Gram-negative Xtra plate format (GNX2F). All strains demonstrating resistance or reduced susceptibility to one of the four carbapenems were subjected to carbapenememase activity detection using the RAPIDEC CARBA NP test, Modified Hodge test and carbapenem inactivation methods. Results: A total of one hundred and forty isolates representing fourteen bacterial species were isolated from 140 non-duplicated specimens. Clinical specimens included urine samples (96/140, 68.57%), sputum (15/140, 10.71%), surgical wound swabs (18/140, 12.85%), foot swabs from diabetes mellitus (DM) patients (6/140, 4.29%), ear swabs (3/140, 2.14%) and wound swabs (2/140, 1.43%). Thirty-four (24.29%) isolates demonstrated resistance to at least one of the four carbapenems with Klebsiella pneumoniae representing 73.53% (25 isolates) of all carbapenem resistant species, followed by 8.82% for Pseudomonas aeruginosa (3 isolates), 5.88% for both Proteus mirabilis (2 isolates) and Escherichia coli (2 isolates) and 2.94% for both Citrobacter koseri (1 isolate) and Rahnella aquatilis (1 isolate). The other isolates were either susceptible or cephalosporinase producers. Conclusion: This study has revealed the high rate of carbapenem resistance amongst Libyan patients and emphasizes the crucial need for accurate screening, identification and susceptibility testing to prevent further spread of nosocomial and community acquired resistance. This may be achieved through the establishment of antibiotic stewardship programmes along with firm infection control practices.National Research Foundation of South Africa; Libyan GovernmentWeb of Scienc